山羊抗小鼠 IgG 二級抗體, DyLight 488 標誌

DyLight 488, Goat Anti-Mouse IgG

貨號

A23210

包裝

100 μl
500 μl

廠牌

Abbkine

產品類別

二級抗體 (Secondary antibody)、多株抗體 (Polyclonal antibody)、流式細胞實驗用抗體 (FCM antibody)、免疫細胞化學染色實驗用抗體 (ICC antibody)、免疫螢光染色實驗用抗體 (IF antibody)

產品簡介

本產品為帶有 DyLight 488 螢光標定之山羊抗小鼠 IgG 二級抗體，可廣泛應用於流式細胞分析 (flow cytometry, FCM)、細胞免疫螢光染色 (ICC/IF) 等實驗。超過 300 篇期刊文獻採用，其中包含《Signal Transduction and Targeted Therapy》《Molecular Cancer》《Cancer Discovery》等頂尖期刊論文，品質穩定可靠。

DyLight 屬於新一代高效能螢光染劑，具備大幅提升的螢光亮度與卓越的抗光漂白特性。在免疫螢光實驗中，DyLight 488 抗體結合物的螢光強度通常優於 Cy2 與 FITC 標定抗體，且與 Alexa Fluor 488 標定抗體相當，能提供極佳的訊噪比與清晰的成像結果。

本產品使用完整小鼠 IgG 分子作為免疫原 (immunogen)，並經由固相化小鼠 IgG (H&L) 親和層析純化製備。抗體純度經 SDS-PAGE 分析確認達 95% 以上。本產品除了可用於偵測完整小鼠 IgG 分子外，亦可辨識免疫球蛋白之重鏈 (heavy chain) 與輕鏈 (light chain)。本產品對非免疫球蛋白之血清蛋白不具反應性，但在特定情況下可能與其他物種之免疫球蛋白產生交叉反應。

Fig.1 DyLight fluorescent dyes are a new family of dyes with improved brightness. DyLight 488-antibody conjugates are brighter than Cy2 and FITC conjugates and similar in brightness to Alexa Fluor 488 conjugates. DyLight 549-antibody conjugates shows brighter influoresence than TRITC conjugates. Also, DyLight 594-antibody conjugates are noticeably brighter than Alexa 594 conjugates, and much brighter and more water soluble than Texas Red conjugates.
DyLight fluorescent dyes show improved photostability as well. DyLight 488 conjugates fade less than FITC and Cy2 conjugates in mounting media indicating that the DyLight 488 molecule is inherently more photostable in epifluorescence microscopes. DyLight 549 conjugates are about as photostable as Alexa 555 conjugates and slightly more photostable than Cy3 conjugates.
Fig.2 DyLight fluorescent dyes show improved photostability as well. DyLight 488 conjugates fade less than FITC and Cy2 conjugates in mounting media indicating that the DyLight 488 molecule is inherently more photostable in epifluorescence microscopes. DyLight 549 conjugates are about as photostable as Alexa 555 conjugates and slightly more photostable than Cy3 conjugates.
To use the DyLight Fluors with fluorescent imagers, use a spectral line of the blue laser diode for DyLight 405, a cyan (488 nm) laser for DyLight 488, a green (526 nm) laser for DyLight 550 and 594, and a red (633 nm) laser for DyLight 633 and 650. The DyLight 680, 755 and 800 fluors are compatible with laser- and filter-based infrared imaging instruments that emit in the 700 nm, 750 nm and 800 nm region of the spectrum, respectively.
Fig.3 To use the DyLight Fluors with fluorescent imagers, use a spectral line of the blue laser diode for DyLight 405, a cyan (488 nm) laser for DyLight 488, a green (526 nm) laser for DyLight 550 and 594, and a red (633 nm) laser for DyLight 633 and 650. The DyLight 680, 755 and 800 fluors are compatible with laser- and filter-based infrared imaging instruments that emit in the 700 nm, 750 nm and 800 nm region of the spectrum, respectively.

應用實驗

FCM, ICC, IF

反應物種 (Reactivity)

小鼠

宿主物種 (Host species)

山羊

免疫原 (Immunogen)

小鼠 IgG 全分子

同種型 (Isotype)

IgG

建議稀釋比例

1:50-1:1000

抗體標記

DyLight 488

濃度

0.5 mg/ml

儲存緩衝液 (Storage buffer)

PBS，pH 7.4，含 0.02% 疊氮化鈉 (sodium azide)、1% 牛血清白蛋白 (BSA) 和 50% 甘油 (glycerol)。

儲存條件

自出貨日起，可於 -20°C 穩定保存一年。建議原包裝瓶首次解凍後，於開蓋前先離心片刻，再開啟分裝保存使用，以避免反覆凍融。

產品說明書

A23210 DyLight 488, Goat Anti-Mouse IgG
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