Lightning-Link® Streptavidin 抗體標定試劑盒

Streptavidin Conjugation Kit - Lightning-Link®

貨號

包裝

3 x 10 μg
100 μg
3 x 100 μg
1 mg

廠牌

產品簡介

Abcam Streptavidin Conjugation Kit - Lightning-Link® 提供了一種簡便快速的方法，可將鏈黴親和素 (Streptavidin) 標定至抗體或其他含有游離胺基的蛋白質、胜肽分子上，標定產物可直接應用於 Western Blot、ELISA、免疫組織化學染色 (IHC) 等實驗。套組採用 Lightning-Link® 直接標定技術，僅需將修飾劑 (Modifier) 加入待標定抗體，再以此溶液回溶凍乾的 Streptavidin 試劑小瓶，即可啟動標定反應；動手操作時間約 30 秒，3.5 小時左右即可完成標定，全程無需任何純化步驟，抗體回收率可達 100%。

主要特色

操作簡便快速，套組採用 Lightning-Link® 直接標定技術，僅需將添加有 Modifier 的待標定抗體與凍乾的 Streptavidin 試劑混合，即可透過離胺酸 (lysine) 等游離胺基進行共價標定，動手操作時間僅約 30 秒，整體反應時間在 3.5 小時左右（標定反應約 3 小時＋中止反應 30 分鐘）。
全程無需純化步驟，抗體回收率可達 100%，標定完成後即可直接應用於 Western Blot、ELISA、IHC 等實驗，無需額外處理。

Single-molecule localization microscopy. Pereira, Pedro M., et al used Streptavidin Conjugation Kit - Lightning-Link® (ab102921) as part of examining super-resolution fluorescent probes for Single-molecule localization microscopy. They used the kit to conjugate Streptavidin to anti-mouse secondary antibody for use in fixed-cell imaging. D, Pre-acquisition snapshot (WF) and SMLM reconstruction (STORM) of β-tubulin immunolabelling with B3S9L SB (B3S9L-ATTO555-BHQ2) and control probe (B3S9L-ATTO555). Scale bars are 5 μm. E, Schematic representation of the strategy used to perform β-tubulin immunolabelling (left), measurement of Full With Half Maximum (FWHM) and distances between structures on the highlighted regions in D. — **Single-molecule localization microscopy.** Pereira, Pedro M., et al used Streptavidin Conjugation Kit - Lightning-Link^® (ab102921) as part of examining super-resolution fluorescent probes for Single-molecule localization microscopy. They used the kit to conjugate Streptavidin to anti-mouse secondary antibody for use in fixed-cell imaging. D, Pre-acquisition snapshot (WF) and SMLM reconstruction (STORM) of β-tubulin immunolabelling with B3S9L SB (B3S9L-ATTO555-BHQ2) and control probe (B3S9L-ATTO555). Scale bars are 5 μm. E, Schematic representation of the strategy used to perform β-tubulin immunolabelling (left), measurement of Full With Half Maximum (FWHM) and distances between structures on the highlighted regions in D. *Image from Pereira et al., Traffic, 21(5):375-385; doi: 10.1111/tra.12728. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/*

實驗注意事項

樣本緩衝液 pH 值須介於 6.5–8.5，相容成分包括：Tris（濃度應 ≤50 mM / 0.6%）、BSA（≤0.1%）、甘油 (glycerol，≤50%)、疊氮化鈉 (Sodium Azide，≤0.1%)、磷酸鉀、氯化鈉、蔗糖、檸檬酸鈉、海藻糖等。緩衝液不可含有 Thimerosal (Thiomersal)、Proclin、Glycine、Arginine、Glutathione 等成分，以免干擾標定反應。更多樣本緩衝液細部條件說明，可參照下方表格或產品說明書。
僅適用於已純化的抗體樣本；若樣本中含有其他蛋白質、胜肽或游離胺基分子，可能會競爭消耗標定試劑，影響標定效率。抗體樣本理想濃度為 1 mg/mL，若濃度不足（< 0.5 mg/ml）或過高（> 5 mg/ml），建議先進行濃縮或稀釋處理。
標定完成的產物於 4°C 保存可穩定達 18 個月；若需長期保存，建議加入 50% 甘油作為冷凍保護劑，並存放於 -20°C。實際最適合的保存條件仍視個別抗體的穩定性而定，建議透過實驗確認。

Compatible buffer constituents
50mM / 0.6% Tris¹	0.1% BSA	50% glycerol
0.1% sodium azide	PBS	Potassium phosphate
Sodium chloride	HEPES	Sucrose
Sodium citrate	EDTA	Trehalose

¹ Tris buffered saline is almost always ≤ 50 mM / 0.6%

Incompatible buffer constituents
Thiomerosal	Proclin	Glycine
Arginine	Glutathione	DTT

套組內容物

Item	3 x 10 µg	100 µg	3 x 100 µg	1 mg	Storage temperature
Streptavidin mix	3 vials	1 vial	3 vials	1 vial	-20°C
Modifier reagent	1 vial	1 vial	1 vial	1 vial	-20°C
Quencher reagent	1 vial	1 vial	1 vial	1 vial	-20°C

產品說明書

ab102921 Streptavidin Conjugation Kit - Lightning-Link®