GAPDH 小鼠單株抗體

Anti-GAPDH Mouse Monoclonal Antibody (2B5)

貨號

ABL1020

包裝

50 μl
200 μl
200 μl×5

廠牌

Abbkine

產品類別

一級抗體 (Primary antibody)、單株抗體 (Monoclonal antibody)、Loading Control 抗體 (Loading Control antibody)、WB 實驗用抗體 (WB antibody)、IF 實驗用抗體 (IF antibody)、IHC-P 實驗用抗體 (IHC-P antibody)

產品簡介

本產品為高專一性單株抗體，可應用於西方墨點法 (Western blot, WB)、免疫螢光染色 (IF) 及石蠟包埋免疫組織化學染色 (IHC-P) 實驗，以偵測 GAPDH 蛋白。

目標蛋白簡介

甘油醛-3-磷酸脫氫酶 (glyceraldehyde 3-phosphate dehydrogenase, GAPDH) 是一種分子量約 37 kDa 的酵素，可催化糖解作用 (glycolysis) 中的第六步反應，於葡萄糖分解與細胞能量代謝過程中扮演重要角色。除了這項已被廣泛建立的代謝功能外，近年研究亦發現 GAPDH 參與多種非代謝性生物過程，包括轉錄活化 (transcription activation)、細胞凋亡 (apoptosis) 啟動、內質網至高基氏體 (ER-to-Golgi) 的囊泡運輸，以及快速軸突運輸 (fast axonal transport) 或軸漿運輸 (axoplasmic transport) 等。

在 WB 實驗中，由於 GAPDH 在多數組織與細胞中具有較高且相對穩定的表現量，因此常作為 loading control 內參蛋白，用以校正樣本間的上樣差異，提升實驗數據的可靠性與可比較性。

應用實驗

IF, IHC-P, WB

Western blot analysis (1:10000) of GAPDH expression in Rat brain (lane A), HeLa cell lysate (lane B), Mouse brain (lane C), Rabbit muscle (lane D), Chicken muscle (lane E) and Pig heart (lane F) with Anti-GAPDH Mouse Monoclonal Antibody (2B5).
Fig.1 Western blot analysis (1:10000) of GAPDH expression in Rat brain (lane A), HeLa cell lysate (lane B), Mouse brain (lane C), Rabbit muscle (lane D), Chicken muscle (lane E) and Pig heart (lane F) with Anti-GAPDH Mouse Monoclonal Antibody (2B5).
Immunohistochemical analysis of paraffin-embedded human colon tissue. 1, GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.
Fig.2 Immunohistochemical analysis of paraffin-embedded human colon tissue. GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded mouse heart tissue. 1, GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.
Fig.3 Immunohistochemical analysis of paraffin-embedded mouse heart tissue. GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.
Fig.4 Immunohistochemical analysis of paraffin-embedded rat kidney tissue. GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.
Immunofluorescence analysis of human colon tissue. GAPDH Monoclonal Antibody (2B5) (red) was diluted at 1:400 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
Fig.5 Immunofluorescence analysis of human colon tissue. GAPDH Monoclonal Antibody (2B5) (red) was diluted at 1:400 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
Immunofluorescence analysis of mouse liver tissue. GAPDH Monoclonal Antibody (2B5) (red) was diluted at 1:400 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
Fig.6 Immunofluorescence analysis of mouse liver tissue. GAPDH Monoclonal Antibody (2B5) (red) was diluted at 1:400 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
Western blot analysis of Pig muscle tissue, GAPDH Monoclonal Antibody (2B5) was diluted at 1:10000 (25°C, 3h).
Fig.7 Western blot analysis of Pig muscle tissue. GAPDH Monoclonal Antibody (2B5) was diluted at 1:10000 (25°C, 3h).
Western blot analysis of HeLa, GAPDH Monoclonal Antibody (2B5) was diluted at 1:10000 (25°C, 3h).
Fig.8 Western blot analysis of HeLa. GAPDH Monoclonal Antibody (2B5) was diluted at 1:10000 (25°C, 3h).

反應物種 (Reactivity)

人、小鼠、大鼠、兔、雞、狗、倉鼠、猴、豬、綿羊、酵母菌

宿主物種 (Host species)

小鼠

免疫原 (Immunogen)

合成胜肽

同種型 (Isotype)

IgG1

建議稀釋比例

  • IF/IHC-P 1:400
  • WB 1:10000-1:100000

濃度

1 mg/ml

儲存緩衝液 (Storage buffer)

PBS，pH 7.4，含 0.02% 疊氮化鈉 (sodium azide) 和 50% 甘油 (glycerol)。

儲存條件

自出貨日起，可於 -20°C 穩定保存一年。建議原包裝瓶首次解凍後，於開蓋前先離心片刻，再開啟分裝保存使用，以避免反覆凍融。

產品說明書

ABL1020 Anti-GAPDH Mouse Monoclonal Antibody (2B5)
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